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Meridian Bioscience 5x mytaq reaction buffer
5x Mytaq Reaction Buffer, supplied by Meridian Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+mytaq+reaction+buffer/mytaq+reaction+buffer/10__1007_slash_s11557___025___02059___2-52-13-17
Average 90 stars, based on 1 article reviews
5x mytaq reaction buffer - by Bioz Stars, 2026-09
90/100 stars

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Polymerase Chain Reaction:

Article Title: Prevalence and intensity of a rickettsiales-like organism in cultured pleasure oyster, crassostrea corteziensis, from nayarit, mexico.
Article Snippet: Fastidious endosymbiotic Rickettsiales-like organisms (RLOs) have been observed in the digestive diverticula of the cultured pleasure oyster (Crassostrea corteziensis) from Nayarit, Mexico since 2007.. In a few mollusk species, these bacteria have been associated with mortality events and production losses.. The type of relationship between the RLOs and the pleasure oyster is largely unknown and further investigations are needed to determine if these bacteria warrant management concern in C. corteziensis.

Article Title: Primary antibody response after influenza virus infection is first dominated by low-mutated HA-stem antibodies followed by higher-mutated HA-head antibodies
Article Snippet: .. The second HC PCR reaction was performed with 2 μl DNA from first HC PCR and the following PCR reaction mix: 0.2 μl forward and reverse multiplex primer mix (equimolar diluted), 0.1 μl MyTaq DNA polymerase (5 U/μl, Meridian Bioscience), and 5x MyTaq reaction buffer in a final reaction volume of 20 μl for 1 min at 95°C, 50 cycles of 95°C for 15 s; 55°C for 15 s; 72°C for 45 s, and a final step of 72°C for 10 min. ..

Article Title: Pangenomes-identified singletons for designing specific primers to identify bacterial strains in a plant growth-promoting consortium.
Article Snippet: Background The use of plant growth-promoting microorganisms represents a sustainable way to increase agricultural yields and plant health.. Thus, the identification and tracking of these microorganisms are determinants for validating their positive effects on crops.. Pangenomes allow the identification of singletons that can be used to design specific primers for the detection of the studied strains.

Article Title: Capturing the fungal diversity hidden in Eastern Cape dairy pastures
Article Snippet: PrepMan Ultra Sample Preparation Reagent (Thermo Fisher Scientific, USA) was used for Fusarium and other hyaline fungi following the manufacturer’s instructions. .. PCR amplifications were prepared in 25 μL total volumes containing 5 μL BioLine 5X MyTaq Reaction Buffer (Meridian BioScience, USA), 0.50 μL of each primer (10 μM), 17.85 μL demineralised sterile water and 0.15 μL BioLine MyTaq DNA Polymerase (Meridian BioScience, USA). ..

Article Title: Exploring genetic variation in saffron (Crocus sativus L.) accessions through two-locus DNA barcoding
Article Snippet: Saffron (Crocus sativus L.) remains among the most costly spices worldwide due to its invaluable properties.. Traditional breeding methods are restricted by saffron’s male sterility and clonal multiplication, which may result in genetic deterioration and make genetic diversity within saffron populations cryptic.. To overcome this cryptic diversity, we conducted a comprehensive genetic analysis of sixteen saffron accessions from Morocco, France, Greece, and Italy using two universal DNA barcodes: ITS2 and trnH-psbA.

Amplification:

Article Title: Prevalence and intensity of a rickettsiales-like organism in cultured pleasure oyster, crassostrea corteziensis, from nayarit, mexico.
Article Snippet: Fastidious endosymbiotic Rickettsiales-like organisms (RLOs) have been observed in the digestive diverticula of the cultured pleasure oyster (Crassostrea corteziensis) from Nayarit, Mexico since 2007.. In a few mollusk species, these bacteria have been associated with mortality events and production losses.. The type of relationship between the RLOs and the pleasure oyster is largely unknown and further investigations are needed to determine if these bacteria warrant management concern in C. corteziensis.

Article Title: Deciphering phylogenetic relationships and genetic diversity of Moroccan saffron (Crocus sativus L.) using SSRg markers and chloroplast DNA SNP markers
Article Snippet: The molecular characterization of C. sativus accessions is of great interest for clonal selection and preservation of valuable resources with the best productive traits and specific patterns related to local products.. To analyze the genetic diversity of Moroccan saffron, fourteen new saffron accessions were evaluated, twelve of which were fromMorocco and two from Kozani in Greece, and the Quercy region of France.. These accessions differ by their geographical distribution and altitudes.

Multiplex Assay:

Article Title: Primary antibody response after influenza virus infection is first dominated by low-mutated HA-stem antibodies followed by higher-mutated HA-head antibodies
Article Snippet: .. The second HC PCR reaction was performed with 2 μl DNA from first HC PCR and the following PCR reaction mix: 0.2 μl forward and reverse multiplex primer mix (equimolar diluted), 0.1 μl MyTaq DNA polymerase (5 U/μl, Meridian Bioscience), and 5x MyTaq reaction buffer in a final reaction volume of 20 μl for 1 min at 95°C, 50 cycles of 95°C for 15 s; 55°C for 15 s; 72°C for 45 s, and a final step of 72°C for 10 min. ..

Sterility:

Article Title: Capturing the fungal diversity hidden in Eastern Cape dairy pastures
Article Snippet: PrepMan Ultra Sample Preparation Reagent (Thermo Fisher Scientific, USA) was used for Fusarium and other hyaline fungi following the manufacturer’s instructions. .. PCR amplifications were prepared in 25 μL total volumes containing 5 μL BioLine 5X MyTaq Reaction Buffer (Meridian BioScience, USA), 0.50 μL of each primer (10 μM), 17.85 μL demineralised sterile water and 0.15 μL BioLine MyTaq DNA Polymerase (Meridian BioScience, USA). ..



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